产品概述

细胞器基因组de novo测序(线粒体和叶绿体)是通过高通量测序技术，获得高质量细胞器基因组信息。细胞器基因组具有高保守性、较低突变率、母系遗传的特性，能够为系统发育、物种鉴定以及生物多样性、群体遗传生物保护等多方面研究提供有价值的信息。

技术参数

备注：具体测序深度还需参考研究目的、基因组复杂度和测序技术方法等信息。三代测序需对细胞器DNA进行富集。

分析流程

元莘优势

帮助客户快速获得原始数据、组装注释结果上传NCBI的收录号

高测序深度，富集更多细胞器基因组信息，避免基因组污染

 丰富的细胞器基因组分析经验，手动校对基因注释结果

一对一的售后为客户文章发表提供专业服务

分析示例

线粒体基因组圈图

叶绿体基因组圈圈图

密码子偏好性分析

叶绿体基因组比较图

叶绿体基因组LSC、SSC和IR区域边界的比较

系统发育树

项目经验

[1] Lingjian Gui,Shaofeng Jiang,Dengfeng Xie,Liying Yu,Yuan Huang,Zhanjiang Zhang,Yingying Liu. Analysis of complete chloroplast genomes of Curcuma and the contribution to phylogeny and adaptive evolution[J]. Gene,2020,732:

[2] Park Inkyu,Song Jun-Ho,Yang Sungyu,Kim Wook Jin,Choi Goya,Moon Byeong Cheol. Cuscuta Species Identification Based on the Morphology of Reproductive Organs and Complete Chloroplast Genome Sequences.[J]. International journal of molecular sciences,2019,20(11):

[3] Qi Wang,Dan Tang,Huayun Guo,Jie Wang,Xinyi Xu,Zhengfei Wang. Comparative mitochondrial genomic analysis of Macrophthalmus pacificus and insights into the phylogeny of the Ocypodoidea & Grapsoidea[J]. Genomics,2020,112(1):

[4] Zhengfei Wang,Qiong Wu,Huayun Guo,Dan Tang,Yuze Bai,Ziqian Wang,Yitao Tao. Next-generation sequencing yields the complete mitogenome of Caridina multidentata and phylogenetic analysis[J]. Mitochondrial DNA Part B,2018,3(1):

[5] Li Gong,Xinting Lu,Zhifu Wang,Kehua Zhu,Liqin Liu,Lihua Jiang,Zhenming Lü,Bingjian Liu. Novel gene rearrangement in the mitochondrial genome of Coenobita brevimanus (Anomura: Coenobitidae) and phylogenetic implications for Anomura[J]. Genomics,2020,112(2):

[6] Feng Jian tong,Guo Ya hong,Yan Cheng rui,Ye Ying ying,Li Ji ji,Guo Bao ying,Lü Zhen ming. Comparative analysis of the complete mitochondrial genomes in two limpets from Lottiidae (Gastropoda: Patellogastropoda): rare irregular gene rearrangement within Gastropoda[J]. Scientific Reports,2020,10(1):